A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
نویسندگان
چکیده
منابع مشابه
A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important to allow a proper case management and implementation of strategies for the containment of virus spread. We show here a sensitive and reproduci...
متن کاملReal-time quantitative reverse transcriptase polymerase chain reaction.
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform. These characteristics have made it the method of choice for minimal residual disease monitoring suc...
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INTRODUCTION Canines are the principal domestic reservoirs of visceral leishmaniasis in both the Old and New World. The development of highly sensitive and quantitative methods, such as real time reverse transcriptase polymerase chain reaction for measurement of canine cytokines has not been exploited in studies of visceral leishmaniasis. OBJECTIVE To standardize the relative quantification o...
متن کاملDetection and quantification in real-time polymerase chain reaction
The estimation of the concentration of an infectious agent in the environment is a key step to trigger an alert when there is a biological threat. This concentration can be obtained trough a quantitative polymerase chain reaction (qPCR). Nevertheless, standard real-time procedure do not address detection delay which is a main concern in alert triggering. Therefore, we propose a method based on ...
متن کاملبررسی بیان ژن vegf در بیماران مبتلا به سرطان مری با استفاده از روش reverse transcriptase real- time polymerase chain reaction
سرطان مری با حدود 386000 مرگ و میر در سال ، ششمین عامل مرگ ناشی از سرطان در جهان است. vegf ، یکی از مهمترین تنظیم کننده های اختصاصی رگ زایی است.ژن vegf در انواع مختلف سرطان مری جهش یافته است. این ژن در مقابل ایجاد جهش در dna وکمبود اکسیژن در تومور فعال شده و تومور به واسطه بیان زیاد این ژن ، رگزایی را آغاز کرده و کمبود اکسیژن خود را از دریافت اکسیژن از خون دریافت کرده و در نهایت متاستاز تومور ر...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2016
ISSN: 1678-8060,0074-0276
DOI: 10.1590/0074-02760150456